PAM Sequences Protect Bacteria from Self-Targeting
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Feng Zhang discusses the protective role of PAM sequences in CRISPR systems.
General HealthContent
Story of claim
PAM sequences prevent CRISPR from mistakenly cleaving the host's own DNA by differentiating bacterial DNA from viral DNA, ensuring safe operation of the CRISPR system.
- Goal: Prevent CRISPR from attacking bacterial DNA.
- Proof: PAM sequences ensure that CRISPR systems do not mistakenly target the host's own DNA.
- Nuances:
- PAM prevents CRISPR from self-targeting.
- PAM is crucial for activation of recognition and cleavage.
- Impact on Life: N/A
Investments
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- Effort: N/A
Risks
Requires precise identification of PAM sequences to prevent self-targeting.
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